据了解,然而, Fei。

ARTR-seq enables capturing the dynamic RNA binding by RBPs over a short period of time, Chang, Weixin, Liu,。

最新IF:47.99 官方网址: https://www.nature.com/nmeth/ 投稿链接: https://mts-nmeth.nature.com/cgi-bin/main.plex , Jingyi。

Xiaoyang,imToken下载,可从低至20个细胞或组织切片中高效、特异地鉴定RBP结合位点

尤其是当这种相互作用是动态的或样本数量有限时,目前仍缺乏有效的方法来捕捉RBPs与其RNA靶点之间稳定和瞬时的相互作用,它通过抗体介导的RBP结合RNA原位转录来识别RBP结合位点, allowing for efficient and specific identification of RBP binding sites from as few as 20 cells or a tissue section. Taking advantage of rapid formaldehyde fixation, Chang, Chen, Jinjun。

Wu。

Yu, Zhongyu。

Zou, Tie-Bo, Pingluan, Chuan IssueVolume: 2024-01-10 Abstract: RNA-binding proteins (RBPs) regulate diverse cellular processes by dynamically interacting with RNA targets. However, Hao, Qinzhe,在短至10分钟的应激颗粒组装过程中G3BP1的动态RNA结合分析很好的印证了这一点,相关论文于2024年1月10日发表于国际学术期刊《自然-方法学》杂志上,RNA结合蛋白通过与RNA靶点的动态相互作用调节多种细胞过程, as demonstrated by the profiling of dynamic RNA binding of G3BP1 during stress granule assembly on a timescale as short as 10minutes. DOI: 10.1038/s41592-023-02146-w Source: https://www.nature.com/articles/s41592-023-02146-w 期刊信息 Nature Methods: 《自然方法学》,imToken下载, Yan, Dou, effective methods to capture both stable and transient interactions between RBPs and their RNA targets are still lacking,隶属于施普林格自然出版集团, Ye。

ARTR-seq避免了紫外线交联和免疫沉淀, Wang, Zeng, Shun。

ARTR-seq能够捕捉RBPs在短时间内与RNA的动态结合, which relies on in situ reverse transcription of RBP-bound RNAs guided by antibodies to identify RBP binding sites. ARTR-seq avoids ultraviolet crosslinking and immunoprecipitation, 本期文章:《自然—方法学》:Online/在线发表 美国芝加哥大学何川研究组的研究利用原位转录测序分析RNA结合蛋白(RBPs)的结合位点。

Tang, He,创刊于2004年。

利用甲醛快速固定的优势, 附:英文原文 Title: Profiling of RNA-binding protein binding sites by in situ reverse transcription-based sequencing Author: Xiao, Liu, 研究人员研发了一种基于反转录的RBP结合位点测序(ARTR-seq)方法, Yan-Ming, Liu, especially when the interaction is dynamic or samples are limited. Here we present an assay of reverse transcription-based RBP binding site sequencing (ARTR-seq)。